Regulatory

Part:BBa_J100126:Design

Designed by: Joe Zhou   Group: Campbell M Lab   (2013-09-12)


pMekA mutated, repressed by glucose


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to shorten the pMekA promotor sequence form the front and back by more than 30 nucleotides. This may cause the promoter to lose its normal function. pMekA is repressed by glucose. The glucose concentration required to repress the promoter is 0.2%.

Source

http://link.springer.com/content/pdf/10.1007%2Fs00253-013-5030-7.pdf

References